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Sunday, August 9, 2020 | History

2 edition of Interrelationship between sterol and phospholipid homeostasis in cultured fibroblasts found in the catalog.

Interrelationship between sterol and phospholipid homeostasis in cultured fibroblasts

Petra Leppamäki

Interrelationship between sterol and phospholipid homeostasis in cultured fibroblasts

by Petra Leppamäki

  • 32 Want to read
  • 37 Currently reading

Published by Åbo Akademis Förlag in Åbo .
Written in English

    Subjects:
  • Cholesterol -- Physiological effect.,
  • Sterols.,
  • Lipids.,
  • Fibroblasts.

  • Edition Notes

    StatementPetra Leppamäki.
    SeriesActa Academiae Aboensis. Ser. B, Mathematica et physica -- vol. 62, no. 1, Acta Academiae Aboensis -- v. 62, nr. 1.
    The Physical Object
    Pagination52 p. :
    Number of Pages52
    ID Numbers
    Open LibraryOL22548001M
    ISBN 109517651147

      The function of a cell membrane is to act as a boundary between the inside of the cell and its surrounding environment. It is made up almost entirely of a phospholipid bilayer and proteins. Molecules that are water soluble, or dissolve in water, are unable to pass through the phospholipid bilyer due to the hydrophobic interior.   Fibroblast growth factor is a widely expressed protein with a broad mode of action, including the regulation of carbohydrate and fatty acid metabolism .

    Cornell, R. B., and Horwitz, A. F., , Apparent coordination of the biosynthesis of lipids in cultured cells: Its relationship to the regulation of the membrane sterol:phospholipid ratio and cell cycling, J. Cell Biol. – PubMed CrossRef Google Scholar.   However, the relationship between NTE aging and ER phospholipid homeostasis and how PMSF pretreatment regulates phospholipid homeostasis needs to be further investigated.

      In this study we have identified ORP9, a cholesterol-binding protein that partitions between the ER and Golgi apparatus, as a potential regulator of sterol homeostasis in the secretory pathway. Similar to OSBP, ORP9L has PH-, FFAT-, and sterol-binding domains that regulate partitioning between the ER and Golgi apparatus. The NE is comprised of two concentric, closely opposed lipid bilayers, the outer and inner nuclear membranes (ONM and INM, respectively). 4, 18 The ONM is continuous with the rough endoplasmic reticulum (rER), studded with ribosomes and functions in secretion and lipid biosynthesis. The ribosome-free INM faces the viscous nucleoplasm, and contains a unique spectrum of integral and membrane.


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Interrelationship between sterol and phospholipid homeostasis in cultured fibroblasts by Petra Leppamäki Download PDF EPUB FB2

PlsEtn levels of cultured RCDP fibroblasts correlate with disease severity. (A) Plasmalogen biosynthesis rates were determined by the method of Roscher et al. and are depicted as ratio between the peroxisomal (14 C) and the ER (3 H) steps of biosynthesis.

Typical biosynthesis rates of healthy controls are indicated by the shaded area Cited by: Furthermore, PlsEtn levels of cultured fibroblasts clearly correlated with those in red blood cells of the corresponding patients (Table 1).

Although plasmalogen levels in patient fibroblasts were in accordance with their rate of biosynthesis, there was an unexpected residual amount of PlsEtn in fibroblasts of the severely affected by: 「Interrelationship between sterol and phospholipid homeostasis in cultured fibroblasts」を図書館から検索。カーリルは複数の図書館からまとめて蔵書検索ができるサービスです。.

Adele D'amico, Enrico Bertini, in Handbook of Clinical Neurology, Leukoencephalopathy with dystonia and sterol carrier protein-2 deficiency. Sterol carrier protein-2 is the second peroxisomal thiolase to be identified (MIM + ).

SCP2 contains both a thiolase domain and a sterol carrier protein domain and is the key enzyme in the β-oxidation of pristanic acid and bile acid.

Some amphiphilic drugs, such as UA, progesterone and imipramine, have been described as interfering with intracellular sterol traffic by accumulating lysosomal unesterified cholesterol in fibroblasts and Chinese Hamster ovary cells [52,53]; the movement of cholesterol from the cell surface to the ER is inhibited by these drugs, which also Cited by: Philip L.

Yeagle, in The Membranes of Cells (Third Edition), Sterol Carrier Proteins. A second mechanism for intracellular cholesterol movement is by sterol carrier protein (SCP). SCP is widely found in cells including in the lumen of the endoplasmic reticulum.

Most biological functions of vitamin A, however, involve the activation of ligand-dependent transcription factors. This hormonal function of vitamin A gained tremendous scientific interest with the discovery of two vitamin A receptors that are members of the nuclear receptor -trans retinoic acid (atRA) is a high-affinity ligand for RAR, while 9-cis retinoic acid (9cRA) and the.

Frolov et al. () have observed that sterol exchange between biological membranes is highly enhanced by the sterol carrier protein SCP2.

In good agreement with these in vitro studies, Puglielli et al. () have shown a requirement for SCP2 in sterol transport from the ER to the PM of cultured fibroblasts. Their results pointed to a. Request PDF | Methods for Monitoring ABCA1-Dependent Sterol Release | Releasing sterols to the extracellular milieu is an important part of sterol homeostasis in cells and in the body.

ATP-binding. 1. Introduction. Oxysterols exhibit several biological activities, including the regulation of cholesterol homeostasis and cytotoxicity.

Members of the ATP-binding cassette (ABC) transporter family have been implicated in a multitude of cell functions, including sterol homeostasis in a variety of tissues. A Phospholipid is a type of lipid whose molecule has a hydrophilic "head" containing a phosphate group, and two hydrophobic "tails" derived from fatty acids, joined by a glycerol residue.

The phosphate group can be modified with simple organic molecules such as choline, ethanolamine or serine. Lange, Y, Swaisgood, MH, Ramos, BV, Steck, TL.

Plasma membranes contain half the phospholipid and 90% of the cholesterol and sphingomyelin in cultured human fibroblasts. J Biol Chem ;– PubMed Google Scholar. Cholesterol is an important modulator of the structure and function of mammalian cell membranes.

The effect of cholesterol is mediated through the interactions between the sterol and the phospholipid components of the membrane. Recently, we have reported a correlation between the relative affinity cholesterol have for different phospholipid components and the degree to which the sterol can.

Therefore, dehydroergosterol (DHE), a naturally occurring fluorescent sterol, was incorporated into cultured L-cell fibroblasts. Two PM markers, the enhanced cyan fluorescent protein (ECFP-Mem. Abstract. A wide range of C 27 to C 30 oxysterols, many of which are products of cholesterol autoxi ation, are potent inhibitors of the enzyme 3-hydroxymeqy4glutaryl-coenzyme A (HMG-CoA) reductase in mammalian cells.

1–4 Because HMG-CoA reductase is an important regulatory enzyme in the biosynthesis of isoprene units the oxysterols can suppress the biosynthesis of several isoprenoid. What Triglycerides, Phospholipids & Sterols Do for the Body.

Triglycerides, phospholipids and sterols are the most common members of the lipids family, the class of nutrients made up primarily of dietary fats. Lipids are naturally occurring compounds that all share one. Insight to the fundamental interrelationship between calcium and lipid homeostasis was first chronicled in Evolutionary Biology, Cell–Cell Communication and Complex Disease.

Further research will solidify the utility of focusing on the advent and roles of cholesterol in eukaryotic evolution, extending from unicellular to multicellular. While transbilayer movement of sterols in synthetic membranes and red blood cells is rapid, in yeast, the general diffusion of membrane components is slow, and sterol movement between leaflets.

The cultured skin fibroblast was the experimental model in which the principles of the regulation of intracellular cholesterol homeostasis were identified and extrapolated to all the other cells. phosphate-containing charged chemical structure that is a component of a phospholipid.

Phosopholipids contains two regions. polar and nonpolar regions. sterol found in animal foods and made in the body;required for bile acid and steroid hormone synthesis enzyme produced in the salivary glands that hydrlyzes ester linkages between fatty.

Cholesterol plays an essential role in normal embryogenesis and perturbations in its de novo synthesis are responsible for organ malformations in the cholesterol biosynthesis defects.

Ten distinct inherited disorders have been linked to different enzyme defects in the isoprenoid/cholesterol biosynthetic pathway: mevalonic aciduria, hyperimmunoglobulinemia syndrome, squalene synthase .For whole-cell cholesterol, the rate constants for efflux to LP-AI and LP-AI/AII were: /h and /h, respectively, with Fu5AH cells; /h and /h with GM human skin fibroblasts.

We synthesized a family of sterol-modified glycerophospholipids (SML) in which the sn-1 or sn-2 position is covalently attached to cholesterol and the alternative position contains an aliphatic chain.

The SML were used to explore how anchoring cholesterol to a phospholipid affects cholesterol behavior in a bilayer. Notably, cholesterol in the SML retains the membrane condensing properties of.